CJC-1295 DAC

Peptides like growth hormone secretagogues and analogues are marketed for muscle growth and recovery but lack clinical evidence for supraphysiological use.; Potential risks include cardiovascular strain, insulin resistance, dyslipidemia, and psychiatric instability.; Unregulated supply chains lead to mislabeled or contaminated products, exacerbating health dangers.; Prevalence data on recreational use, especially among younger individuals, is critically lacking.

A refined sample preparation combining ultrafiltration and solid-phase extraction was developed for GHRH and analogs in urine.; The method was fully validated according to WADA guidelines, including selectivity, robustness, and matrix effects.; Achieved LODs of 0.5 ng/mL and LOIs of 0.5-0.75 ng/mL for target peptides in urine.; The method is suitable for both screening and confirmation analyses in anti-doping testing.

Liquid chromatography-high-resolution mass spectrometry (>100,000 FWHM) enables initial testing of prohibited peptides in blood with simplified sample preparation.; Mixed-mode solid-phase extraction allows generic extraction of multiple peptide classes, including insulins, GHRHs, IGFs, and MGFs, from blood.; Blood analysis detects intact peptide hormones at higher concentrations than urine, facilitating detection.; The method fulfills WADA TD2022 MRPL requirements and demonstrated proof of principle with post-administration samples.

Limits of detection were 5-25 pg/mL and limits of identification were 25-50 pg/mL.; Recoveries ranged from 59-115%, with similar sensitivity to immuno-affinity purification but lower costs.; Sermorelin and its metabolite degraded rapidly at temperatures >4°C and pH <7.; The method can be expanded to cover emerging peptide drugs and metabolites without specific antibodies.

CBD and carboxy-THC act as extracellular ligands for GHS-R1a, strongly promoting ghrelin binding.; Cannabinoids promoted GHR/GHS-R1a interaction, while synthetic GHR mimetics acted as competitive inhibitors.; The assay displayed good linearity and sensitivity for CBD and carboxy-THC in 1:4 diluted urine at 5-30 ng/mL.; GHR mimetics showed no interference at concentrations up to 100 ng/mL, and results were validated by GC-MS/MS.

Nineteen major in vitro metabolites of four GHRH analogs were identified and synthesized as reference materials.; A sensitive LC-MS/MS method was developed for detecting parent peptides and metabolites in urine.; Detection limits for target peptides were generally 1 ng/ml or less, meeting WADA performance limits.

CJC-1295 is a peptide drug that stimulates growth hormone production and forms long-lasting conjugates with plasma proteins.; Conjugated CJC-1295 is difficult to detect by mass spectrometry due to low abundance and high molecular weight.; The new LC-MS/MS method successfully confirmed CJC-1295 after immuno-affinity capture and tryptic digestion.; The method detected CJC-1295 at concentrations as low as 180 pg/mL in equine plasma.

CJC-1295 covalently links to plasma proteins via a maleimidopropionic acid group, evading standard mass spectrometry detection.; The I-PCR assay detects the CJC-1295-protein conjugate at concentrations as low as 0.8 pg/mL.; A screening threshold of 50 pg/mL was established to differentiate CJC-1295 from endogenous equine GHRH.; The assay effectively identified CJC-1295 in equine plasma samples after administration to thoroughbred race horses.

What is CJC-1295 DAC?

CJC-1295 with Drug Affinity Complex (DAC) is a synthetic analog of growth hormone-releasing hormone (GHRH) engineered to extend its half-life via covalent attachment to a drug affinity complex (DAC), which binds to serum albumin. This modification allows for sustained, multi-day release of the peptide, resulting in pro

How does CJC-1295 DAC work?

Albumin-binding (DAC) GHRH analog producing sustained, multi-day elevation of GH and IGF-1.

What is the research status of CJC-1295 DAC?

CJC-1295 DAC is currently classified as discontinued, with 35 research references on record. This is for research purposes only and is not medical advice.

What is the half-life of CJC-1295 DAC?

The reported half-life of CJC-1295 DAC is 6-8 days.