Epitalon

experimental

Also known as: Epithalon, AEDG, Ala-Glu-Asp-Gly

**Mechanism of Action** Epitalon (Ala-Glu-Asp-Gly) is a synthetic tetrapeptide derived from the pineal polypeptide extract epithalamin. Its primary proposed mechanism involves upregulation of telomerase activity, thereby elongating telomeres and potentially delaying cellular senescence. Additionally, Epitalon is reported to restore age-related declines in pineal melatonin secretion by modulating the hypothalamic-pituitary-gonadal axis, leading to improved circadian rhythmicity and antioxidant defense in aging models. **Key Research Findings** Preclinical studies in rodents demonstrate that Epitalon extends median lifespan, reduces incidence of age-associated pathologies (e.g., cataracts, tumors), and enhances immune function. In vitro, the peptide increases telomerase activity in human fibroblasts and retinal pigment epithelial cells. A small human pilot study (n=12) suggested improved sleep quality and melatonin profiles in elderly subjects, though larger controlled trials are lacking. The peptide’s effects on telomere length in humans remain unconfirmed. **Clinical Relevance** Epitalon is not approved for clinical use by any regulatory agency. Its experimental status limits application to laboratory research on aging mechanisms, telomere biology, and pineal function. No validated dosing or safety data exist for human use beyond preliminary studies. For research purposes only — not medical advice.

Key data

Category
Anti-Aging & Longevity
Molecular weight
390.35 g/mol
Molecular formula
C14H22N4O9
CAS number
307297-39-8
Administration
subcutaneous, intramuscular
Research status
experimental
References
63
Tags
telomerase, bioregulator, pineal

Research & studies

Therapeutic peptides in gerontology: mechanisms and applications for healthy aging
Frontiers in aging · 2026 · PubMed

Nine peptides were identified spanning diverse aging interventions: metabolic restoration, telomere biology, dermal regeneration, tissue repair, neuroprotection, growth hormone modulation, and sexual function.; FDA-approved agents demonstrated robust safety profiles from large-scale trials, while non-approved peptides lack long-term safety data and systematic validation.; Significant knowledge gaps include optimal dosing regimens, combination therapy effects, and biomarkers for monitoring efficacy.

Epitalon increases telomere length in human cell lines through telomerase upregulation or ALT activity
Biogerontology · 2025 · PubMed

Epitalon increased telomere length in normal cells through hTERT mRNA expression and telomerase upregulation.; In cancer cells, telomere extension occurred via ALT (Alternative Lengthening of Telomeres) activation.; Only minor ALT activity was observed in normal cells, indicating cancer-specificity.; Dose-dependent telomere length extension was confirmed by qPCR and immunofluorescence analysis.

The Antioxidant Tetrapeptide Epitalon Enhances Delayed Wound Healing in an in Vitro Model of Diabetic Retinopathy
Stem cell reviews and reports · 2025 · PubMed
Overview of Epitalon-Highly Bioactive Pineal Tetrapeptide with Promising Properties
International journal of molecular sciences · 2025 · PubMed

Epitalon is a tetrapeptide (Ala-Glu-Asp-Gly) derived from Epithalamin.; It exhibits geroprotective and neuroendocrine effects via antioxidant, neuroprotective, and antimutagenic actions.; It influences melatonin synthesis, interleukin-2 mRNA levels, thymocyte mitogenic activity, and enzymes like AChE, BuChE, and telomerase.; Physico-chemical and structural studies of Epitalon are limited despite extensive biological research.

Epitalon-activated telomerase enhance bovine oocyte maturation rate and post-thawed embryo development
Life sciences · 2025 · PubMed
Epitalon protects against post-ovulatory aging-related damage of mouse oocytes in vitro
Aging · 2022 · PubMed
AEDG Peptide (Epitalon) Stimulates Gene Expression and Protein Synthesis during Neurogenesis: Possible Epigenetic Mechanism
Molecules (Basel, Switzerland) · 2020 · PubMed
[Peptides and CCL11 and HMGB1 as molecular markers of aging: literature review and own data]
Advances in gerontology = Uspekhi gerontologii · 2014 · PubMed

Frequently asked questions

What is Epitalon?

**Mechanism of Action** Epitalon (Ala-Glu-Asp-Gly) is a synthetic tetrapeptide derived from the pineal polypeptide extract epithalamin. Its primary proposed mechanism involves upregulation of telomerase activity, thereby elongating telomeres and potentially delaying cellular senescence. Additionally, Epitalon is report

How does Epitalon work?

Khavinson tetrapeptide reported to activate telomerase and normalize pineal melatonin rhythms in aging models.

What is the research status of Epitalon?

Epitalon is currently classified as experimental, with 63 research references on record. This is for research purposes only and is not medical advice.

What is the molecular weight of Epitalon?

Epitalon has a molecular weight of approximately 390.35 g/mol (formula C14H22N4O9).

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